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DNA-Microarray Analyses (Affymetrix Platform)

Technology

Affymetrix offers several types of arrays. An overview of the current product line and explanations of the technology can be found on the manufacturer's homepage.

Procedure / service

The GTL offers a variety of services for Affymetrix arrays in cartridges. We are happy to assist in the selection of the appropriate platform / array as well as the planning of experiments. The latter point is of particular concern to us in terms of the most effective possible data analysis. As a result, you will receive the raw data in the following formats:

* .jpg  => contains the information of the scanned image
* .DAT => contains the intensity values ​​of the individual pixels of the scanned image
* .CEL => contains the intensity values ​​for the individual probes on the array, calculated from the DAT file. This file is usually used to evaluate the data.

A mere hybridization and scan service is available upon request. In this case, arrays as well as the synthesized and fragmented sample material is provided by you. We take care of the last step consisting of hybridization, washing and scanning.

We are offering analysis of your processed array data as well. If you are interested in a cooperation, please .

Sample Requirements

Sample requirements are dependent on the service chosen:
For the hybridization and scanning service, we need fully fragmented cRNA. The procedure can be found in the protocols for the various assays.
For the full-service we start with total RNA, which is provided by you. The requirements for the samples can be found in the PDF requirements for RNA sample material, which can be found in the download section.

Notes on planning an array experiment

When planning the experiment, biological replicates should be provided for each condition. We recommend a minimum of 4 replicates per condition to be examined. This way, on the one hand, one obtains more robust statistics and, on the other hand, is protected against possible hybridization failures.
Microarray experiments should conform to the MIAME standard (Minimum Information About a Microarray Experiment). Further information can be found here.


Contact Person:


(0211) 81 - 1 04 75


(0211) 81 - 1 43 67
 

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