Workflows/Sample Requirements - QC- Analyses

The GTL is happy to assist you with quantitative and qualitative QC analysis of your DNA and RNA samples. For this purpose, various devices are available on the GTL. Quantitative analyzes can be performed by using spectrophotometry (NanoDrop) as well as nucleic acid-specific employing a fluorometric assay (Qubit).

Qualitative analyzes are performed with a capillary gel electrophoresis system. We conduct integrity analyzes of RNA samples using either a Bioanalyzer (Agilent Genomics) or a Fragment Analyzer (Advanced Analytical Technologies). The analysis on the Bioanalyzer system is accredited according to DIN EN ISO / IEC 17025: 2005.

The Fragment Analyzer is used for the qualitative analysis of high molecular-weight genomic DNA. Here fragment sizes up to 50,000 bp can be determined. For larger fragments, at least an estimate of the fragment length can be made.

In addition, the success of RNA amplifications, RNA fragmentation in the context of microarray analyses as well as the quality of NGS libraries can be examined with the systems mentioned above.

The current costs for such analyses can be found undefinedhere.

FAQ - QC-Analyses

What are the sample requirements for QC samples?
  • Samples intended for the Bioanalyzer or Fragment Analyzer should be provided in at least 5 μl of water or elution buffer. Please make sure that the concentration has been adjusted according to the type of assay you have ordered! The handling of the samples on site is strictly limited and the individual samples are neither measured nor are further dilutions carried out. We therefore do not take liability for any errors due to incorrect sample concentration.

How long does it take to QC my samples?
  • QC of your samples is usually performed within two working days.

How will the results be provided?
  • QC results will be provided via Email as a PDF or Excel-Sheet after completion of the analysis.

How long are samples stored after analysis?
  • Analyzed samples are stored after the results have been made available for a maximum of five working days at -20 ° C. After this periods, samples will be disposed of. If you wish to keep your samples, please  let us know and pick up your samples after the analysis has been completed.

    GENERALLY VALID: Please provide us with nothing more than an ALIQUOT of your sample

Can prokaryotic & plant samples be analyzed?
  • Yes, it is possible to analyze prokaryotic and plant samples. Please let us know at the time of sample delivery, so that the bioanalyzer can be configured accordingly (for the correct identification of the additional plastid rRNA or the correct identification of the 16S or 23S rRNA and the assignment of a corresponding RIN).

Head of GTL

Prof. Dr. Karl Köhrer

Biologisch-Medizinisches Forschungszentrum (BMFZ) Heinrich-Heine-Universität Düsseldorf Universitätsstr. 1 40225 Düsseldorf
Phone +49 211 81-13165
Fax +49 211 81-11922
Responsible for the content: E-MailRedaktionsteam BMFZ