Qualitative QC-Analyses
For qualitative assessment of nucleic acid samples (DNA and RNA), the GTL has a Fragment Analyzer (AATI) at its disposal. Qualitative assessment of high molecular weight gDNA is carried out on the Femto Pulse. These platforms works with fluorescent dyes and are therefore very sensitive so that even low concentrations of nucleic acids can be analysed.
In order to avoid failures, please pay attention to the observance of the concentration ranges for the respective assay (see below), as the concentrations are usually not checked by us in advance. The minimum sample volume should not be less than 5 μl.
Fragment Analyzer
| Assay | Type | Concentration | Sizing range [bp] | Samples /run |
|---|---|---|---|---|
| DNF-471 Standard Sensitivity RNA Assay | totalRNA | 10 - 500 ng/µl | 200 - 6.000 | 1 - 11 |
| mRNA | 10 - 500 ng/µl | |||
| DNF-472 High Sensitivity RNA Assay | totalRNA | 250 - 5.000 pg/µl | 200 - 6.000 | 1 - 11 |
| mRNA | 500 - 5.000 pg/µl | |||
| DNF-474 High Sensitivity NGS Fragment Analysis Kit | defined Peak | 50 - 500 pg/µl | 100 - 6.000 | 1 - 11 |
| smear analysis | 250 - 5.000 pg/µl | |||
| DNF-464 High Sensitivity Genomic large fragment 50kb DNA Analysis Kit | smear analysis | 50 - 5.000 pg/µl gDNA (Optimum 1ng/µl) | 75 - 48.500 | 1 - 11 |
| DNF-467 genomic DNA 50 kb Kit | smear analysis | 25 - 250 ng/µl | 75 - 60.000 | 1 - 11 |
Femto Pulse
| Assay | Type | Concentration | Sizing range [kbp] | Samples /run |
|---|---|---|---|---|
| gDNA 165 kb (FP-1002) | high molecular weight gDNA | 0,1 - 0,3 ng/µl | 1.3 - 165 | 1 - 11 |
Formalities
Please use our TSM system for ordering. After successful registration, QC-Analyses can be ordered. Please hand in the prepared samples together with an order form ("Blockzettel") (sample information is entered directly in the TSM system) to building 22.07 floor U1, room 61.
Important: Please mention the order number from the TSM system on the order form.
After the analysis is completed, results will be provided via email to the address provided in the TSM as a *.pdf file. In addition, in the case of Bioanalyzer results, you will receive a corresponding *.xad file with the original measurement data. This file format is readable with the Bioanalyzer Expert software, which can be downloaded from the Agilent Homepage.