Long-Read Sequencing

Pacific Biosciences' Single Molecule Real Time (SMRT) sequencing technology can deliver over 20,000 nucleotides, and Nanopore Sequencing from Oxford Nanopore can even sequenced hundreds of kb long reads. This allows a better assembly of the individual DNA pieces after sequencing (de novo assembly), which ideally leads to completely closed genomes. With the advantage of "classical" short read or next generation sequencing techniques (such as those offered by Illumina), the long reads allow for bridging repetitive sequences, uniform coverage of GC-rich regions, and complete sequencing of particular target regions. Although the error rate for individual reads for PacBio is 12-15% and for Nanopore currently about 8%, the random distribution of the errors allows a very high consensus accuracy of the assembled genomes (QV> 50). Furthermore, these technologies allow the detection of m6A and m4C base modifications, which are mainly found in microbial genomes. With any whole genome sequencing of these organisms, the information about base modifications and the corresponding motifs can be bioinformatically analyzed.

The following is a list of the currently available long-read workflows at the GTL. If your desired workflow is not listed here, please .