Workflows/Sample Requirements - Long-Read Sequencing

Pacific Biosciences' Single Molecule Real Time (SMRT) sequencing technology can deliver over 20,000 nucleotides, and Nanopore Sequencing from Oxford Nanopore can even sequenced hundreds of kb long reads. This allows a better assembly of the individual DNA pieces after sequencing (de novo assembly), which ideally leads to completely closed genomes. With the advantage of "classical" short read or next generation sequencing techniques (such as those offered by Illumina), the long reads allow for bridging repetitive sequences, uniform coverage of GC-rich regions, and complete sequencing of particular target regions. Although the error rate for individual reads for PacBio is 12-15% and for Nanopore currently about 8%, the random distribution of the errors allows a very high consensus accuracy of the assembled genomes (QV> 50). Furthermore, these technologies allow the detection of m6A and m4C base modifications, which are mainly found in microbial genomes. With any whole genome sequencing of these organisms, the information about base modifications and the corresponding motifs can be bioinformatically analyzed.

The following is a list of the currently available long-read workflows at the GTL. If your desired workflow is not listed here, please undefinedcontact us.

10X Genomics Workflows & Sample Requirements

FAQ - QC-Analyses

Which documents are required for submitting a long-read order?
  • Before ordering a long-read analysis, you must first undefinedregister with us and upon registration accept our privacy policy. Then you can place an order electronically. In addition to the samples themselves, the following documents are required to process your samples:

    • a completed "Blockzettel" as order form (including cost center and GTL billing account)
    • Sample information sheet (this will be provided automatically when submitting an order)
    • the "undefinedAnalysauftrag NGS", filled in as far as possible (details will be supplemented upon submission of samples)
    • possibly a undefinedrelease according to GenDG.
What is the pricing of long-read analysis at the GTL?
  • Due to the highly different requirements from project to project, prices for NGS orders are always calculated on a project-specific basis. Please undefinedcontact us for a non-binding quote.

What are the sample requirements for long-read samples?
  • The sample requirements for the different workflows and applications can be found on the corresponding pages (see above).

Head of GTL

Prof. Dr. Karl Köhrer

Biologisch-Medizinisches Forschungszentrum (BMFZ) Heinrich-Heine-Universität Düsseldorf Universitätsstr. 1 40225 Düsseldorf
Phone +49 211 81-13165
Fax +49 211 81-11922
Responsible for the content: E-MailRedaktionsteam BMFZ