The GTL is happy to assist you with quantitative and qualitative QC analysis of your DNA and RNA samples. For this purpose, various devices are available on the GTL. Quantitative analyzes can be performed by using spectrophotometry (NanoDrop) as well as nucleic acid-specific employing a fluorometric assay (Qubit).
Qualitative analyzes are performed with capillary gel electrophoresis systems. We conduct integrity analyzes of RNA samples using a Fragment Analyzer (Advanced Analytical Technologies).
The Fragment Analyzer is also used for the qualitative analysis of genomic DNA. Here fragment sizes up to 50,000 bp can be determined. For the analysis of high molecular weight gDNA fragments up to a size of 165 kb can be analyzed on the Femto Pulse system.
In addition, the success of RNA amplifications, RNA fragmentation in the context of microarray analyses as well as the quality of NGS libraries can be examined with the systems mentioned above.
The current costs for such analyses can be found here.
The following options are available for QC
FAQ - QC-Analyses
Samples intended for the Fragment Analyzer should be provided in at least 5 μl of water or elution buffer. Please make sure that the concentration has been adjusted according to the type of assay you have ordered! The handling of the samples on site is strictly limited and the individual samples are neither measured nor are further dilutions carried out. We therefore do not take liability for any errors due to incorrect sample concentration.
QC of your samples is usually performed within two working days.
QC results will be provided via Email as a PDF or Excel-Sheet after completion of the analysis.
Analyzed samples are stored after the results have been made available for a maximum of five working days at -20 ° C. After this periods, samples will be disposed of. If you wish to keep your samples, please let us know and pick up your samples after the analysis has been completed.
GENERALLY VALID: Please provide us with nothing more than an ALIQUOT of your sample
We can of course also examine prokaryotic and plant samples. When submitting the sample, please give us a note so that the Fragment Analyzer can be configured accordingly (for the correct identification of the additional plastid rRNA or the correct identification of the 16S or 23S rRNA and assignment of a corresponding RIN).