Fluorometric measurements
Qubit
The Qubit fluorometer and corresponding assays are available for fluorometric measurements. In such a measurement, nucleic acids (DNA, RNA) are specifically detected. The sample is mixed with a dye. This dye (fluorophore) binds selectively to DNA or RNA, so that contamination (other nucleic acids, phenol residues, absorbing salts, etc ...) does not or only minimally influence the concentration determination.
- Specific, quantitative measurement of DNA and RNA using a fluorometric assay
- The fluorophore binds specifically to the DNA / RNA, allowing a more accurate measurement compared to photometric measurements
- Sample volume: 1 - 20 μl
- available assays:
- dsDNA HS assay - range 0.2 - 100 ng
- RNA HS Assay - Measurement range 5 - 10 ng