Whole Genome Sequencing

In whole genome sequencing, the complete DNA of an organism is sequenced. Depending on the size of the genome, various NGS platforms may be used, with MiSeq (Illumina) being used for small to medium genome sizes (1 Mb to 120 Mb, with an average coverage of 100x). For larger genomes, the NextSeq2000 (Illumina) is a suitable option.

Genomes of Mycobacterium sp. (~ 4.4 to 6.9 Mb, GC content> 65%), Chlamydia sp. (~ 1 Mb) and Enterobacter sp. (~ 5.3 Mb) have been successfully sequenced  on the MiSeq.

The GTL can help with planning and design of an experiment as well as its implementation and data analysis.